Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism

Abstract. Lestari DA, Sutopo, Setiaji A, Kurnianto E. 2023. Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism. Biodiversitas 24: 617-624. This research provides useful information to reflect the genetic structure determine appropriate breeding strategies for Java. The objectives this were investigate relationship (Bos indicus) A total 208 samples collected, which belonged 6 populations Java, namely Rembang, Blora, Kebumen, Semarang, Magelang, Kudus regency. Blood analysis was conducted by Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE) methods separate molecule weight. Results showed 4 locus clearly identified, Albumin (Alb), Post (Pa), Ceruloplasmin (Cp), Amylase-I (Amy-I), formed 9 identified alleles 15 genotypes. All had different allele gene frequency distribution each locus, dominated AlbB (0.525), PaB (0.556), CpF (0.608), Amy-IB (0.639) genotype BB (0.312) Alb loci, (0.429) Pa FS (0.423) Cp loci (0.472) Amy-I. deviation Hardy-Weinberg Equilibrium (HWE) (P<0.05) Kudus, while rest HWE condition. Average heterozygosity distance recent range 0.328-0.529 (for Magelang Kebumen populations, respectively) 0.0057-0.0955 Rembang-Kebumen Magelang-Kebumen population), respectively. In conclusion, high divided into two major clusters.